TOPSAN

The Dhaf4260 gene (uniprot: B8FUJ5) from Desulfitobacterium hafniense encodes a protein of unknown function (DUF364 (PF04016)) encountered in archaea and prokaryotes. The structure reveals a two-domain organization with a PLP-dependent transferase-like fold preceded by a N-terminal domain adopting an enolase N-terminal domain-like fold. Structural and genetic context analysis suggests a possible role in the anaerobic biosynthesis of vitamin B12.[Ref]

OVERALL STRUCTURE 

Dhaf4260 is a two-domain α+β protein (Fig.1).The N-terminal domain (residues 1-102) adopts an enolase N-terminal-like fold characterized by three helices with up-down topology and a three-stranded anti-parallel β-sheet. The C-terminal domain (residues 110-251) adopts a PLP-dependent transferase-like fold characterized by a three-layer α/β/α sandwich topology with a seven-stranded mixed β-sheet...