ACΔ3-6 Stable Knockout HEK293 Cell Line | T6291

abm | ACΔ3-6 Stable Knockout HEK293 Cell Line | T6291

AC3 and AC6 encoded by the ADCY3 and ADCY6 genes, are the most abundant adenylyl cyclase (AC) isoforms in HEK293 cells. ACΔ3/6 Stable Knockout HEK293 Cell Line was generated by knocking out the AC3 and AC6 genes through stable transfection, selection with puromycin (2 mg/ml), and where then determine to exhibit low cAMP levels.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 30 hours

Seeding Density:

N/A

Markers:

Puromycin-resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) cAMP detection kit

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C