ACTB CRISPR Stable HEK293T Cell Line | T6247

abm | ACTB CRISPR Stable HEK293T Cell Line | T6247

β-Actin (ACTB) was integrated into a specific genomic loci using CRISPR/Cas9 in HEK293T cells. The transgene is driven by endogenous promoters to ensure consistent and predictable expression of ACTB. A protein quantification reporter (PQR) was incorporated between the endogenous and RPL13A genes. The PQR allows equimolar production of the endogenous protein, the recombinant protein, and a fluorescent reporter. As a result, expression levels of both the endogenous and recombinant proteins can be continuously monitored using fluorescence.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

25 - 35 hours

Seeding Density:

20,000 - 30,000 cells/cm²

Markers:

RFP

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C