ARL13-B Stably Expressing HEK 293 Cell Line | T6041

abm | ARL13-B Stably Expressing HEK 293 Cell Line | T6041

ARL13-B is a small GTPase belonging to the class of Arf/Arl family within the Ras superfamily of small GTPases involved in diverse cellular functions. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs and mutations in this gene leads to the classical form of Joubert Syndrome. Since ARL-13B is linked to a wide range of ciliary processes, this cell line could be helpful to study various processess related to cilium formation, function and transport.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent mixed with suspension

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

N/A

Markers:

Puromycin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 1500rpm for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO2.

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1. Real Time PCR was used to quantify ARL13 B gene expression in immortalized cell line. 2. Puromycin selection. 2. Puromycin selection

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C