BVRA Stable Knockout MCT Cell Line | T6281

abm | BVRA Stable Knockout MCT Cell Line | T6281

BVRA (biliverdin reductase A) was knocked out of the mouse proximal tubule cells (MCT) using sgRNAs inserted into the GuideIt-zsGreen plasmid. The plasmid was transfected and clones were selected via FACS sorting for fluorescence. Knockout was determined by qPCR and Western blot analysis. Studies have shown the loss of BVRA is associated with fat accumulation in livers of mice models. Therefore, BVRA Stable Knockout MCT Cell Line is useful to study the effects of BVRA and its association with obesity, increased lipid availability, and lipotoxicity.

Biosafety:

II

Organism:

Mouse (M. musculus)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 hours

Seeding Density:

7,000 - 8,000 cells/cm²

Markers:

Loss of biliverdin reductase A (BVRA), no drug resistance have been reported

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) qPCR; 2) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C