c-Kit-D816F Stable Ba-F3 Cell Line | T3053

abm | c-Kit-D816F Stable Ba-F3 Cell Line | T3053

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system. Gain-of-function mutations of the receptor have been observed in malignancies such as myeloid leukemia, gastrointestinal stromal tumors, and testicular carcinoma. While loss-of-function mutations were found in defects relating to hematopoiesis, melanogenesis, and gametogenesis. The c-Kit/D816F-expressing Ba/F3 cells (with GNNK- isoform) has a substitution of the aspartic acid residue at position 816 to a phenylalanine. The cells are resistant to Imatinib (Gleevec), an inhibitor of the c-Kit juxtamembrane mutations. Together with the wild-type c-Kit stable Ba/F3 cells (

Biosafety:

I

Organism:

Mouse

Source Organ:

Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfected with pMSCVpuro vector carrying mutant D816F human c-Kit construct

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

N/A

Quality Control:

1) Immunoprecipitation and western blotting for presence of c-Kit; 3) PCR analysis

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C