Cas9 Expressing Doxycycline Inducible HeLa (cTT20) Cell Line | T6175

abm | Cas9 Expressing Doxycycline Inducible HeLa (cTT20) Cell Line | T6175

This HeLa cell line expresses Cas9 when induced by doxycycline. A PiggyBac transposon system was used to introduce Cas9. This parental cell line is applicable for work requiring human CRISPR/Cas9 knockout cells. Lack of pre-existing guide RNA enables freedom to introduce custom gRNA for desired gene targets.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

40,000 - 50,000 cells/cm²

Markers:

Neomycin resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 50% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Immunofluorescence

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C