CNBP Stably Expressing HEK 293 Cell Line | T6212

abm | CNBP Stably Expressing HEK 293 Cell Line | T6212

The CCHC-type Zinc Finger Nucleic Acid Binding Protein (CNBP/ZNF9) is conserved in eukaryotes and essential for embryonic development in mammals. It has been implicated in transcriptional as well as post-transcriptional gene regulation; however, its nucleic acid ligands and molecular function remain elusive. CNBP interacts with mature mRNAs in G-rich regions and it has been proposed to enhance translation by resolving stable structures on mRNAs. It has recently been reported that LAST, a c-Myc-inducible long noncoding RNA, cooperates with CNBP to promote CCND1 mRNA stability in human cells leading to the abundance of cyclin D1, an important regulator of cell cycle.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent mixed with suspension

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

N/A

Markers:

Puromycin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

1. Pre-warm complete media in a 37°C waterbath. 2. Remove the cryopreserved vial from the liquid nitrogen storage tank. 3. Thaw the cells quickly by placing the lower half of the vial into the 37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells. 4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media. 5. Centrifuge cells at 1500rpm for 3 minutes to pellet. 6. Aspirate out the media, leaving cell pellet undisturbed. 7. Re-suspend pellet in fresh culture medium and plate in new culture vessel. 8. Incubate cultures at 37°C, 5% CO2.

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1. Real Time PCR was used to quantify CNBP gene expression in immortalized cell line. 2. Puromycin selection

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C