CPS I Stable Expressing CHO (CHO-CPS45-22) Cell Line | T6177

abm | CPS I Stable Expressing CHO (CHO-CPS45-22) Cell Line | T6177

Carbamoyl phosphate synthetase I (CPS I), a key enzyme in the urea cycle, was introduced to CHO cells by transfection with calcium phosphate to enable permanent CPS I gene expression. This cell line is suitable for use in the study of the urea cycle. Expression of CPS I results in decreased ammonium ion production, making these cells ideal for research regarding the affect of ammonia levels on cell growth and productivity.

Biosafety:

II

Organism:

Hamster

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

CPS I, G418 (500 μg/mL)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII medium available at abm

Preservation:

1. Freeze Medium: Prigrow VIII with 40% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C