DJ-1 CRISPR Stable Knockout HeLa Cell Line | T6181

abm | DJ-1 CRISPR Stable Knockout HeLa Cell Line | T6181

DJ-1 CRISPR Stable Knockout HeLa Cell line was established using the CRISPR/Cas9 system and validated by immunoblotting with anti-DJ-1 antibody and by genomic DNA sequencing. The functions of DJ-1 are not yet fully understood, though studies show that the protein may play a role in mitigating oxidative stress, particularly in brain cells. It is also thought that DJ-1 may act as a chaperone protein. Mutations in PARK7, the gene encoding the DJ-1 protein, are associated with early-onset Parkinson disease. This cell line is suitable for research regarding the functions of DJ-1 including its role in the development of Parkinson disease.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

40,000 - 50,000 cells/cm²

Markers:

Genomic DJ-1 knock-out

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Immunoblotting; 2) Genomic DNA sequencing; 3) STR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C