EGFR-K721A Stable Expressing CHO Cell Line | T6256

abm | EGFR-K721A Stable Expressing CHO Cell Line | T6256

CHO cells were transfected with a plasmid carrying N1-EGFR(K721A)-YFP to establish the EGFR-K721A Stable CHO Cell Line. The cells were selected with 800 μg/ml G418, then sorted through FACS for fluorescence-positive cells. EGFR-K721A is EGFR mutated to have an inactive tyrosine kinase domain. May be used alongside with EGFR Stable CHO Cell Line (abm,

Biosafety:

II

Organism:

Chinese Hamster (C. griseus)

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 28 hours

Seeding Density:

20,000 - 40,000 cells/cm²

Markers:

YFP, G418 resistance, EGFR K721A mutant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) FACS sorting

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C