Fat-1 Stable A549 Cell Line | T6262

abm | Fat-1 Stable A549 Cell Line | T6262

Omega-3 polyunsaturated fatty acids ( ω3-PUFAs) are known to inhibit carcinogenesis, tumour growth and metastasis, whereas omega-6 polyunsaturated fatty acids ( ω6-PUFAs) stimulate their effects. The FAT1 gene of C. elegans encodes aω3-desaturase that catalyses the conversion ofω6-PUFAs toω3-PUFAs, increasing the amount ofω3-PUFAs. Fat-1 Stable A549 Cell Line expresses the full-length FAT1 gene from C. elegans through stable transfection. These cells are applicable for studies investigating the effects of endogenousω3-polyunsaturated fatty acids.

Biosafety:

II

Organism:

Human

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

18 - 26 hours

Seeding Density:

20,000 - 25,000 cells/cm²

Markers:

Fat-1, G418 resistance (0.2 mg/mL)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) RT-PCR

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C