FGFR1 Expressing Stable Ba-F3 (ZNF) Cell Line | T6145

abm | FGFR1 Expressing Stable Ba-F3 (ZNF) Cell Line | T6145

Fusing a kinase domain to a homodimerization domain such as the ZNF domain can result in activated kinase. The FGFR1 Expressing Stable Ba/F3 (ZNF) Cell Line expresses activated human FGFR1 tyrosine kinase (ZNF-FGFR1 in MSCV vector). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival and as a result, the cells can be propagated even in the absence of IL-3.

Biosafety:

I

Organism:

Mouse

Source Organ:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Round

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 - 30 hours

Seeding Density:

50, 000 - 100, 000 cells/ml

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

FGFR1 tyrosine kinase (ZNF-FGFR1) via retroviral transduction

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

FACS

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C