FLAG-HnRNP-L Stable Expressing HEK293 Cell Line | T6250

abm | FLAG-HnRNP-L Stable Expressing HEK293 Cell Line | T6250

In humans, actively transcribed genes are mediated by KMT3a. Within that protein complex is a eukaryotic specific subunit, heterogeneous nuclear ribonucleoprotein L (HnRNP-L). HnRNP-L is a member of a large family of RRM domain containing RNA-binding proteins. It has been implicated in various RNA-related processes, particularly in mediating selective exon inclusion during alternative splicing and facilitating polyadenylation. As such, this cell line can be used to study the role HnRNP-L plays in regulating chromatin modification, post transcriptional events, and co-transcriptional pre-mRNA processing.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Flag-tagged HnRNP-L

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) MALDITOF/TOF; 2) RNase treatment and co-immunoprecipitation; 3) HKMT Assay; 4) RNAi knockdown of KMT3a and HnRNP-L; 5) Immunofluorescence

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C