FLAG-LBMBT1 Stable Expressing HEK293 Cell Line | T6249

abm | FLAG-LBMBT1 Stable Expressing HEK293 Cell Line | T6249

Malignant-brain-tumor (MBT) protein L3MBTL1 is in a complex comprised of core histones, histone H1b, HP1g, and Rb. In humans, L3MBTL1 is a known transcriptional repressor, which requires its three MBT domains for silencing. This cell line is useful to study the transcriptional repression linked to the formation and maintenance of facultative heterochromatin and the mechanism behind Histone-Methylation Dependent Chromatin Lock.

Biosafety:

II

Organism:

Human

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Polyonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Flag-Tagged LBMBT1

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 70% Complete growth medium with 20% FBS and 1% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Peptide Affinity Chromatography
2) Chromatin-Compaction Assay
3) Electron microscopy was conducted on samples using a (JEOL 1200 EX) transmission electron microscope
4) ChIP Sequencing

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C