FLAG-SNAIL Stable Expressing HEK293 Cell Line | T6290

abm | FLAG-SNAIL Stable Expressing HEK293 Cell Line | T6290

The SNAIL family have been associated with EMT (epithelial-mesenchymal transition) events, and tumour progression. FLAG-SNAIL Stable Expressing HEK293 Cell Line was developed through stable transfection with the flag-tagged, full-length AJUBA cDNA in the pcDNA3.1 vector into HEK293 cells. The transfected cells were selected with G418. The cells may be used to further study SNAIL and its association with tumor development and progression.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

30,000 - 50,000 cells/cm²

Markers:

G418 -resistant, Flag-SNAIL

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C