FLNC Stable Knockout Human A2780 Cell Line | T9503

abm | FLNC Stable Knockout Human A2780 Cell Line | T9503

FLNC Stable Knockout Human A2780 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the FLNC gene (NM_001458) using the following guide RNA sequence: GCTGCTTGGGCGTCTGTTTG. FLNC is expressed in cardiac and skeletal muscles and thus can be used to study associated human cardiac diseases such as hypertrophic cardiomyophathy and restrictive cardiomyopathy. This cell line is sequenced verified to cause frameshift mutation.

Biosafety:

II

Organism:

Human

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

T3-9

Passage Number:

6

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at ABM

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 1.0 µg/ml of puromycin (G264) was used for clonal selection; 2) FLNC gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C