GAB1 Stable Knockout Human 8505c Cell Line | T9501

abm | GAB1 Stable Knockout Human 8505c Cell Line | T9501

GAB1 Stable Knockout Human 8505c Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the GAB1 gene (NM_002039) using the following guide RNA sequence: CCTGGCAGCTCTTTACAAGC. GAB1 gene has important implications for B cell malignancies and this cell line is useful in the study of branching tubulogenesis. This cell line is sequenced verified to be biallelic knockout.

Biosafety:

II

Organism:

Human

Source Organ:

Thyroid

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

C302

Passage Number:

5

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 0.8 µg/ml of puromycin (G264) was used for clonal selection; 2) GAB1 gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C