GFAT1 CRISPR Stable Knockout H1299 Cell Line | T6199

abm | GFAT1 CRISPR Stable Knockout H1299 Cell Line | T6199

Glutamine:fructose-6-phosphate amidotransferase (GFAT1) is involved in the hexosamine biosynthesis pathway (HBP) which have been reported to have a critical role in the insulin resistance and progression of cancer. GFAT1 is the rate limiting step in the pathway, thus is a target of interest for study.
GFAT1 CRISPR Stable Knockout H1299 Cell Line was generated to study the effects of GFAT1. The cells may be rescued with treatment with exogenous GlcNAc (N-Acetylglucosamine) to maintain the propagation and glycosylation.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Lung

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 hours

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C