GOT2 Knockout Stable HEK293 Cell Line (Clone A6) | T6425

abm | GOT2 Knockout Stable HEK293 Cell Line (Clone A6) | T6425

CRISPR/Cas9 was used to introduce a disruption of the GOT2 gene in HEK293 cells. The resulting CRISPR/Cas9 GOT2-knockout HEK293 cell line is compound heterozygous for 205del/190_206del/203_206dup.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial

Clones:

N/A

Passage Number:

N/A

Population Doner:

30 - 40 hours

Seeding Density:

30,000 - 50,000 cells/cm²

Markers:

lacZ-Zeocin, FRT integration site, GOT2 was knocked-out using pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid # 48138)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation: