HeLa Stably Expressing HXBc2 tat (tat-III) Cell Line | T6020

abm | HeLa Stably Expressing HXBc2 tat (tat-III) Cell Line | T6020

HeLa Stably Expressing HXBc2 tat (tat-III) Cell Line is an effective tool for the trans-activation of transfected HIV-1 LTR. An in vitro cell line which can continuously propagate for a reliable source of source material for experiments; it is a valuable tool for research in HIV and the role of HCBc2 (gp120).

Biosafety:

II

Organism:

Human

Source Organ:

Cervical

Growth Properties:

Suspension (horse serum)/Adherent (FBS)

Morphology:

Round, Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Transfection with a Moloney-based retroviral vector containing a SalI-BamHI segment from the HIV-1 genomic clone pHXBc2

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line isPrigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) 400 µg/ml neomycin (G418) for selection; 2) Western blot was preformed for gp120.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C