hSSAO-VAP-1 A7r5 Stable Cell Line | T3155

abm | hSSAO-VAP-1 A7r5 Stable Cell Line | T3155

SSAO (semicarbazide-sensitive amine oxidase) has been associated with diabetes, Alzheimer’s, atherosclerosis, and stroke. Thus, it is necessary to have a reliable source of cells which expresses the desired protein for studies in its role and activity in pathways. The hSSAO/VAP-1 A7r5 Stable Cell Line stably expresses SSAO/VAP-1 in the plasma membrane, and releases SSAO into the culture medium. This cell line does not exhibit MAO-A nor MAO-B activities. It is a valuable source for studies in SSAO/VAP-1 membrane-bound form enzyme as a therapeutic target.

Biosafety:

II

Organism:

Rat

Source Organ:

Aorta, Thoracic Smooth Muscle

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

Human SSAO/VAP-1

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

N/A

Quality Control:

1) Selection via antibiotic G418; 2) Western blot used to determine presence of SSAO; 3) Activity of SSAO, MAO-A, and MAO-B was detected by radiochemical methods

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C