Immortalized Human Bronchial Epithelial Cells-hTERT/BMI | T0498

abm | Immortalized Human Bronchial Epithelial Cells-hTERT/BMI | T0498

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying hTERT and BMI genes

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

Doubling time of approx. 23 hours

Seeding Density:

5,000 - 7,500 cells per cm10²

Markers:

Cytokeratin

Donor Age:

55

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

Use of Gelatin Coating Solution (0.1%) is required for cell adhesion to the culture vessels. The base medium for this cell line is Prigrow X series medium available at abm. TM0498.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Real Time PCR was used to quantify hTERT and BMI gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C