Immortalized Human Bronchial Smooth Muscle Cells | T0493

abm | Immortalized Human Bronchial Smooth Muscle Cells | T0493

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

20 - 30 hours

Seeding Density:

20,000 - 30,000 cells/cm10²

Markers:

smooth muscle alpha actin

Donor Age:

27

Donor Gender:

Male

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, sodium pyruvate to a final concentration of 1 mM, L-glutamine to a final concentration of (2 mM), nonessential amino acids to a final concentration of 1% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C