Immortalized Human Cardiomyocytes - CDK4, hTERT, & BMI | T0539

abm | Immortalized Human Cardiomyocytes - CDK4, hTERT, & BMI | T0539

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying CDK4, hTERT, and BMI1 genes

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Ventricle

Organ Type:

Heart

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

15 - 25 hours

Seeding Density:

10,000 - 20,000 cells/cm10²

Markers:

Puromycin resistance, GATA-4, ACTN2, ACTN3 & MYH2

Donor Age:

33 years old

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow X medium available at abm. TM0539.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C