Immortalized Human Cranial Suture Progenitor Cells (iSuPs) | T0697

abm | Immortalized Human Cranial Suture Progenitor Cells (iSuPs) | T0697

Immortalization Method:

Immortalized with retrovirus containing SV40 T antigen, flanked by FRT or loxP sites, selected with hygromycin B

BioSafety Level:

II

Organism:

Human

Species:

Human

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

CD73, CD105 (endoglin), CD90 (Thy-1), CD166 (ALCAM), BMPR-II, CD117 (c-kit), CD29 (Integrin β1)

Donor Age:

15-17 months

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C