abm immortalized cells
Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES) | T0284
- SKU:
- T0284
- Availability:
- 5 to 7 Days Shipment
Description
abm | Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES) | T0284
Immortalization Method:
Conditional immortalization by tetracyclin-controlled transduction with retrovirus carrying v-myc genes
BioSafety Level:
II
Organism:
Homo sapiens
Species:
Human
Source Organ:
8-week-old fetal human ventral mesencephalon
Organ Type:
Brain
Growth Properties:
Adherent
Morphology:
Flattened|Dendritic processes
Passage Number:
N/A
Population Doubling:
30 - 40 hours
Seeding Density:
50,000 - 100,000 cells/cm10²; cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5
Markers:
DAT, VMAT-2, TH, α-SYN, β-III tubulin
Donor Age:
8 week
Donor Gender:
undetermined
Donor Ethnicity:
N/A
Propagation:
Grow the cells in culture vessel pre-coated with 50 μg/ml poly-L-ornithine (PLO) (TM062) and 1 μg/ml fibronectin (EMD Millipore; Cat. FC010) in H2O for at least 3 hours at 37°C.
The base medium for this cell line is Advanced DMEM/F12 (Gibco; 12634010). To make the complete growth medium, add the following components to the base medium: 1x N2 supplement (ThermoFisher Scientific), 2 mM L-glutamine and 40 ng/ml recombinant bFGF (Z101455).
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Quality Control:
mRNA and protein expression levels of various markers pre and post differentiation are verified by RT-PCR and western blotting.
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C