Immortalized Human Lymphoblastoid Cells – EBV (KMS-9) | T0132

abm | Immortalized Human Lymphoblastoid Cells – EBV (KMS-9) | T0132

The KMS-9 cells express HLA-DR on its surface. It also exhibit slight expression of the CD19 B cell marker, but on the other hand, it is negative for CD20. Another lyphoblastoid cell line, KMS-15 (Cat#T0133), in contrast, expresses both CD19B and CD20 cell marker.

Immortalization Method:

Epstein-Barr Virus (EBV)

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Peripheral blood

Organ Type:

Blood

Growth Properties:

Suspension

Morphology:

Round|Clusters

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C