Immortalized Mouse alpha-GalA-/o Schwann Cells (1089C1) | T0744

abm | Immortalized Mouse alpha-GalA-/o Schwann Cells (1089C1) | T0744

Immortalization Method:

Spontaneous immortalization

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Spindle-shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

S100, laminin, p75NTR, GFAP, globotriaosylceramide (Gb3)

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR; 2) Immunocytochemical analysis; 3) Assaying of α-galactosidase activity and protein determination

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C