Immortalized Mouse Basophil Cells (EML C.1) - SV40 | T0679

abm | Immortalized Mouse Basophil Cells (EML C.1) - SV40 | T0679

Immortalization Method:

Immortalized with retroviral vector LRARα403SN

BioSafety Level:

II

Organism:

Mus musculus

Species:

Mouse

Source Organ:

Bone Marrow

Organ Type:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Irregular

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Sca-1, B220, ter119

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow V medium available at abm. to a final concentration of 20%, Penicillin/Streptomycin Solution to a final concentration of 1%, and 200 ng/mL rat stem cell factor (Amgen).
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated serum for cell culture unless specified otherwise.

Quality Control:

1) Southern and Northern analyses; 2) PCR detection of D-J rearrangement; 3) Flow cytometry; 4) Colony assays

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C