Immortalized Mouse Cardiac Endothelial Cells (MCEC) | T0722

abm | Immortalized Mouse Cardiac Endothelial Cells (MCEC) | T0722

Immortalization Method:

Immortalized via infection with lentivirus containing hTERT and SV40-T antigen

BioSafety Level:

II

Organism:

Mouse

Species:

Mouse

Source Organ:

Heart

Organ Type:

Heart

Growth Properties:

Adherent

Morphology:

Endothelial-like

Passage Number:

N/A

Population Doubling:

20 - 30 hours

Seeding Density:

15,000 - 20,000 cells/cm10²

Markers:

SV40-T, hTERT, PECAM-1, VE-cadherin, von Willebrand Factor

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III available at abm. to a final concentration of 5%, 1X GlutaMAX (Gibco 35050061), 1X HEPES (Gibco 15630080), 40 µg/mL heparin, 25 µg/mL endothelial cell growth supplement (Sigma E2759), and Penicillin/Streptomycin Solution to a final concentration of 1%. This cell line needs to be cultured in gelatin coated flasks.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Cell incubation and prostaglandin assays; 2) Immunofluorescence studies; 3) Western blot; 4) rtPCR; 5) Immunohistochemistry; 6) Permeability studies

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C