Immortalized Mouse Dermal Papilla Cells (iDP6) | T0720

abm | Immortalized Mouse Dermal Papilla Cells (iDP6) | T0720

Immortalization Method:

Infection with retrovirus containing SV40 large T antigen

BioSafety Level:

II

Organism:

Mouse

Species:

Mus musculus

Source Organ:

Skin

Organ Type:

Skin

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

24 - 48 hours

Seeding Density:

25,000 - 50,000 cells/cm10²

Markers:

FGF7, SOX2, TBX18, α-SMA

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow VIII available at abm. to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, Sodium Pyruvate Solution to a final concentration of 1%, MEM Non-essential Amino Acid Solution (Sigma) to a final concentration of 1%, and Recombinant Mouse FGF2 to a final concentration of 10 ng/mL.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR; 2) Immunocytochemistry staining; 3) Alkaline phosphatase staining; 4) Western blot

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C