Immortalized Mouse Embryo Neural Stem Cells (MuNSC) | T0817

abm | Immortalized Mouse Embryo Neural Stem Cells (MuNSC) | T0817

abm, Cat. No, supplemented with B27 supplement (Gibco) to a final concentration of 1%, epidermal growth factor to a final concentration of 10 ng/mL, basic fibroblast growth factor-2) to a final concentration of 10 ng/mL, 100U/mL penicillin, and 100 ug/mL streptomycin.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Monolayer cells expressing specific neural lineage markers can be differentiated from neurospheres when grown in regular Prigrow III propagation media.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Neural stem cells can be used to study neurodegenerative diseases and research neural repair mechanisms.

Immortalization Method:

Immortalized by using the 3T3 protocol.

BioSafety Level:

II

Organism:

Mouse (M. musculus)

Species:

Mouse

Source Organ:

Brain

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

GFAP, NESTIN, and MBP

Donor Age:

13.5 dpc embryo

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, L-glutamine to a final concentration of 2 mM and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 3-4 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Senescence β-galactosidase staining kit; 2) Western blot; 3) Immunocytochemistry; 4) Cell proliferation assays; 5) Wound-healing assay; 6) Metaphase chromosome preparation

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C