Immortalized Mouse Hepatic Stellate Cells - SV40 | T0688

abm | Immortalized Mouse Hepatic Stellate Cells - SV40 | T0688

Immortalization Method:

Transfection of pCMV Simian Virus 40 Large T antigen

BioSafety Level:

II

Organism:

C57/Bl6 WT mouse

Species:

Mouse

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

35 - 45 hours

Seeding Density:

20,000 - 40,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% with Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) ³[H]-thymidine Cell Proliferation Assay

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C