Immortalized Mouse Lung Clara Cells-Conditionally (C22) | T0092

abm | Immortalized Mouse Lung Clara Cells-Conditionally (C22) | T0092

in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate.

C22 resembles closely to primary clara cells and constitutively expresses and secretes surfactant proteins SP-A, SP-B, SP-D and CC10. This cell line is useful in studying lung epithelial physiology, as well as studying the role of CC10 in pulmonary inflammation and viral infection.

Immortalization Method:

Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)

BioSafety Level:

II

Organism:

H-2Kb-tsA58 transgenic mouse

Species:

Mouse

Source Organ:

Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

1) Presence of CC10, SP-A, SP-B and absence of SP-C confirmed by RT-PCR; 2) Synthesis and secretion of CC10, SP-A, SP-B and SP-D proteins confirmed by immunoblotting

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 100 U/ml γ-IFN

Quality Control:

N/A

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C