Immortalized Mouse MyD88^-/- Hepatic Stellate Cells | T0690

abm | Immortalized Mouse MyD88^-/- Hepatic Stellate Cells | T0690

-/- mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen. Myeloid differentiation factor 88 (MyD88) is an adaptor protein downstream of the TLR4 pathway. LPS activation of the Immortalized Mouse MyD88^-/- Hepatic Stellate Cells expressing WT TLR4 has shown almost no change in NF-κB activation. The Immortalized Mouse MyD88^-/- Hepatic Stellate Cells are useful in studies focusing on liver fibrosis, especially in the role of the inflammatory TLR4 pathway activation in scar tissue formation. In addition, the cells can be used in studies of cellular survival regulation, since TLR4 activation has been linked to apoptosis-resistance.

Immortalization Method:

pCMV Simian Virus 40 Large T antigen

BioSafety Level:

II

Organism:

MyD88^-/- mouse

Species:

Mouse

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

25-35 hours

Seeding Density:

10,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) ³[H]-thymidine Cell Proliferation Assay

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C