Immortalized Rat Retinal Capillary Endothelial Cells-SV40 | T9097

abm | Immortalized Rat Retinal Capillary Endothelial Cells-SV40 | T9097

Immortalization Method:

Serial passaging and infection with Lentivirus carrying SV40 antigen gene

BioSafety Level:

II

Organism:

Rattus norvegicus

Species:

Rat

Source Organ:

Eye

Organ Type:

Eye

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

P10

Population Doubling:

24-28 hours

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C