JWA Stable Knockout Human MHCC97L Cell Line | T9504

abm | JWA Stable Knockout Human MHCC97L Cell Line | T9504

JWA Stable Knockout Human MHCC97L Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the JWA gene (NM_006407) using the following guide RNA sequence: AGAGCAGGTTGCTCACTACG. JWA gene is associated with cytoskeleton and has been reported to regulate intracellular concentrations of taurine and glutamate. As such, this cell line may be useful to study membrane trafficking. This cell line is sequenced verified to be biallelic knockout.

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

2C-C7

Passage Number:

5

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 1.5 µg/ml of puromycin (G264) was used for clonal selection; 2) JWA gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C