KtyI Stable Expressing Mouse Keratinocyte Cell Line | T6294

abm | KtyI Stable Expressing Mouse Keratinocyte Cell Line | T6294

KtyI Stable Expressing Mouse Keratinocyte Cell Line are spontaneously immortalized cells. The cells express keratins (ktyl) and may be used in conjunction with Ktly Stable Knockout Mouse Keratinocyte Cell Line (T6293) to study the affects of the presence and absence of keratins.

Biosafety:

II

Organism:

Mouse

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

30,000 cells/cm²

Markers:

Ktyl (Keratin)

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is DMEM/Ham’s F12, low Calcium 50µM) Biochrom). To make the complete growth medium, add the following components to the base medium: chelexed fetal calf serum PAA, A15-151) to a final concentration of 10%, 2mM Glutamax Invitrogen, 35050-038), 1X Pyruvate PAA, S11-003), 0.18mM Adenin Sigma, A8626-5G), 10 ng/ml EGF Invitrogen 53003-018), 2.5 µg/ml insulin Sigma-Aldrich, I-9278), 0.5 µl Hydroxycortison Sigma-Aldrich, H4001-1G), 10^-10 M Cholera toxin Sigma,C-8052), and Penicillin/Streptomycin Solution

Preservation:

1. Freeze Medium: Complete growth medium with 20% chelexed-FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C