LIN28B Stable Knockout Human A2780 Cell Line | T9506

abm | LIN28B Stable Knockout Human A2780 Cell Line | T9506

LIN28B Stable Knockout Human A2780 Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the LIN28B gene (NM_001004317) using the following guide RNA sequence: TACGGGTAACAGGACCTGGT. LIN28B gene is belongs to the lin-28 family. it is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. This cell line is sequenced verified to be biallelic knockout.

Biosafety:

II

Organism:

Human

Source Organ:

Ovary

Growth Properties:

Adherent

Morphology:

Cobblestone

Clones:

T3-9

Passage Number:

6

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow II medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) 1.0 µg/ml of puromycin (G264) was used for clonal selection; 2) LIN28B gene knockout confirmed via surveryor assay and sanger sequencing of targeted genomic region

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C