mIR-216a Stable Expressing PDL8 HUVEC Cell Line | T6166

abm | mIR-216a Stable Expressing PDL8 HUVEC Cell Line | T6166

Biosafety:

II

Organism:

Human

Source Organ:

Umbilical Vein

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Stable expression of microRNA-216a (mIR-216a). Infected with pre-miR-216a recombinant lentiviruses (Ubi-EGFPMCS-IRES-puromycin). These cells exhibit puromycin resistance and express enhanced green fluorescent protein (EGFP).

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is endothelial cell medium ScienCell). To make the complete growth medium, add the following components to the base medium: fetal bovine serum

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) puromycin selection

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C