mTLR4<sup>---<-sup> Hepatic Stellate Cell Line Stably Expressing hTLR4 | T6104

abm | mTLR4^{---<-sup> Hepatic Stellate Cell Line Stably Expressing hTLR4 | T6104}

mTLR4^-/- Hepatic Stellate Cell Line Stably Expressing hTLR4 was generated via lentiviral transduction with flag-tagged TLR4 Wildtype, and were selected for using 500 μg/mL Zeocin. The cells can be used in studies of cellular survival regulation, as TLR4 activation has been linked to apoptosis-resistance.

Biosafety:

II

Organism:

Mouse

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

Zeocin-resistant

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C