abm drug discovery
NF-κB Reporter CHO Cell Line (EL-1) | T6434
- SKU:
- T6434
- Availability:
- 5 to 7 Days Shipment
Description
abm | NF-κB Reporter CHO Cell Line (EL-1) | T6434
The NF-κB Reporter CHO Cell Line (EL-1) was constructed to express surface CD25 upon IL-1 or TNF stimulation by stable transfection of CHO fibroblast cells with the NF-κB-dependent reporter construct containing endothelial leukocyte adhesion molecule CD25, and the hygromycin vector pCEP4 (without CD14).
Biosafety:
II
Organism:
Chinese Hamster (C. griseus)
Source Organ:
Ovary
Growth Properties:
Adherent
Morphology:
Epithelial-Like
Clones:
N/A
Passage Number:
N/A
Population Doner:
20 - 30 hours
Seeding Density:
20,000 - 40,000 cells/cm2
Markers:
Surface CD25 upon IL-1 or TNF stimulation Hygromycin B and Ciprofloxacin Resistant
Applications:
For Research Use Only
Doner Gender:
N/A
Donor Ethnicity:
N/A
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
Flow cytometry using a FACScan microfluorometer & Western Blot
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C