P-gp Stable Expressing MDCK Cell Line | T6302

abm | P-gp Stable Expressing MDCK Cell Line | T6302

P-gp Stable Expressing MDCK Cell Line are stably over-expressing the MDR1 gene through long term induction with Doxorubicin-HCl. Studies have shown P-gp to restricts oral absorption, thus may impact drug disposition. This cell line is useful for assessing drugs as canine P-gp substrates.

Biosafety:

II

Organism:

Dog (Canine)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

N/A

Markers:

MDR1 (P-gp), Doxorubicin

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is MEM/EBSS HyClone SH30024). To make the complete growth medium, add the following components to the base medium: fetal bovine serum

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C