PD-L1 CRISPR Stable Knockout MC38 Cell Line | T6149

abm | PD-L1 CRISPR Stable Knockout MC38 Cell Line | T6149

PD-L1 CRISPR Stable Knockout MC38 Cell line is derived from a widely used murine colon carcinoma model, MC38. Guide RNAs (gRNA) carried on a gRNA cloning vector were used to target exon 1 of the mouse CD274 gene encoding Programmed death-ligand 1. Successful knockout of PD-L1 was verified by immunefluorescence after cells were subjected in interferon-gamma.

Biosafety:

II

Organism:

Mouse

Source Organ:

Colon

Growth Properties:

Adherent

Morphology:

Epithelial-like, Bipolar

Clones:

N/A

Passage Number:

N/A

Population Doner:

15 - 25 hours

Seeding Density:

5,000 - 20,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

PD-L1 negative

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow V medium available at abm

Preservation:

N/A

Quality Control:

1) Confirmed the Knock out of PD-L1 through Facs analysis ( Figure 1B)

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C