PNKP-FLAG Stable Expressing HEK293 Cell Line | T6126

abm | PNKP-FLAG Stable Expressing HEK293 Cell Line | T6126

PNKP-FLAG Stable Expressing HEK293 Cell Line was established through stable transfection of the human PNKP expressing pTV61 plasmid. Stable clone was selected with 200 µg/ml hygromycin B. PNKP, or polynucleotide kinase 3′ phosphatase, is a bifunctional DNA end-processing enzyme involved in the NHEJ pathway.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Kidney

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

PNKP-FLAG, Hygromycin B

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C