ProClone™ Competent Cells | E003

abm | ProClone™ Competent Cells | E003

ProClone™ Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/µg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone™ Competent Cells the premier choice for both routine and challenging subcloning projects.

Concentration:

N/A

Form:

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Alternate Name:

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Reaction Buffer:

N/A

Reaction:

N/A

Notes:

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Application:

Restriction enzyme digestion of double-stranded DNA

Components:

EcoRV enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer

Inactivation:

65°C for 20 min

Source:

An E.coli strain that carries the modified EcoRV gene from the plasmid J62 pLG74 (L.l. Glatman). The EcoRV enzyme recognizes and cuts the sequence GAT^ATC.

Shipping conditions:

Dry ice

Storage conditions:

Store at -80°C.

Storag Buffer:

N/A