Recombinant Human AMPK (A2/B1/G3) (N-GST/C-His tag) | Z500025

abm | Recombinant Human AMPK (A2/B1/G3) (N-GST/C-His tag) | Z500025

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis . AMPK exists as a heterotrimeric complex composed of a catalytic a subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta.

Functions:

The specific activity of AMPK was determined to be 360 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G3: PRKAG3

Gene Symbol:

AMPK (A2/B1/G3)

Gene ID:

N/A

Accession:

NM_006252, NM_006253, NM_017431

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

92

Molecular Weight 2 (kDA):

65

Molecular Weight 3 (kDA):

108

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.