Recombinant Human AMPK (A2/B2/G1) (C-His tag) | Z500027

abm | Recombinant Human AMPK (A2/B2/G1) (C-His tag) | Z500027

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis . AMPK exists as a heterotrimeric complex composed of a catalytic a subunit and regulatory ß and ? subunits. Binding of AMP to the ? subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKß .

Functions:

The specific activity of AMPK was determined to be 280 nmol/min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G1: PRKAG1, AMPKG, MGC8666

Gene Symbol:

AMPK (A2/B2/G1)

Gene ID:

N/A

Accession:

NM_006252, NM_005399, NM_002733

Formulation:

50mM sodium, pHosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

69

Molecular Weight 2 (kDA):

36

Molecular Weight 3 (kDA):

41

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.