Recombinant Human AMPK (A2/B2/G2) (N-GST/C-His tag) | Z500028

abm | Recombinant Human AMPK (A2/B2/G2) (N-GST/C-His tag) | Z500028

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis . AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta.

Functions:

The specific activity of AMPK was determined to be 65 nmol /min/mg as per activity assay protocol.

Organism:

Human

Source:

Insect cells

Alias:

Subunit A2: PRKAA2, AMPK, AMPK2, PRKAA Subunit B2: PRKAB2, MGC61468 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p

Gene Symbol:

AMPK (A2/B2/G2)

Gene ID:

N/A

Accession:

NM_006252, NM_005399, NM_001040633

Formulation:

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Solubility:

N/A

Appearance:

Liquid

Molecular Weight (kDa):

92

Molecular Weight 2 (kDA):

62

Molecular Weight 3 (kDA):

105

Purity:

70% - 90%

Concentration:

Shipping Condition :

Dry Ice

Storage Condition:

Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.